Using bromothymol blue you wouldn't be able to distinquish between anacid with pH 4 from an acid with pH 5. 1 decade ago. In this experiment, spectrophotometry is employed to measure the pKa of bromothymol blue, an acid-base indicator. They would both appearyellow. Supplier: MP Biomedicals. 2 Answers. Ensure that the solutions are mixed well. Computational methods, such as SQUAD (84) 16 and SPECFIT/32, and some new methods Determination of the pK_a of Bromothymol Blue Use the following graph to determine the molar absorptivity of Bromothymol blue (BTB)? Molar absorptivity ___ (You will be using this value in your experiment.) [10] [11] describe multiwavelength analysis and nonlinear least squares regres-sion methods. Place three small drops of bromothymol blue solution into each of the 5 cuvettes. ,IC10113005EA,IC10113025EA. Molecular Weight 646.36 . pH = pKa - log ( [HIn] / [In-] ) … (2) As a pH indicator,bromothymol blue, for example, would be useful between from about pH6.0 to pH 7.6. It shows that pH=pKa where [ln-]= [Hln] or hwere ( [ln-]/ [Hln])=1 or where log ( [ln-]/ [Hln])=0. Given the equation: y= 48048x- 0.0105, find the molar absorptivity and use this value in your experiment. Description: Bromothymol Blue (BTB) is a pH indicator (pKa approx. Chloroform showed the highest affinity for bromothymol blue, followed by methylene chloride, ethylene dichloride, benzene, toluene, and carbon tetrachloride. PubChem Substance ID 24892083. Anonymous. For this reason it can be electrostatically immobilized on the surface on anion-exchanger resin like Amberlite IRA 401. 2. Explain why the acid - base indicator bromothymol blue turns yellow when added to dilute HCl but blue when added to dilute NaOH. Beilstein/REAXYS Number 6887926 . Meloun et al. MDL number MFCD00077263. Solutions of different concentration of the indicator were used. EC Number 252-169-7. Empirical Formula (Hill Notation) C 27 H 27 Br 2 NaO 5 S . 7.1) that is used as a biological slide vital stain to define cell walls or nuclei under the mic... SDS. Relevance. The indicator (HIn) is a monoprotic acid and we can represent its dissociation as follows: HIn → H+ + In- … (1) The equilibrium expression for such dissociation can be written as. Bromothymol blue, Powder ACS. Other indicators work in different pH ranges. Answer Save. NACRES NA.47 There is a linear relationship between log ( [ln-]/ [Hln]) and pH. Into each of the cuvettes place 3 mL of the solutions of the various pH values (hydrochloric acid, the buffer solutions with pH values 6.0, 7.0 and 8.0, and sodium hydroxide solution). Favorite Answer. Assume a cuvet with a 14.45 mm diameter was used. The pKa can be determined from the spectrophotometric data using different methods. Spectrophotometric determination of the pKa of Bromothymol Blue. Acid - Base indicators are either weak acids or weak bases so they exist as molecules in equilibrium with their ions. Bromothymol blue is an acidic pH chromoionophore where acid to base change corresponds to its neutral to anionic form, respectively. The pKa′ value was determined using a method similar to that of Reilley and Swayer. Bromothymol Blue sodium salt powder Synonym: 3′,3′′-dibromothymolsulfonephthalein sodium salt CAS Number 34722-90-2. Indicator: pH Range: pKa: Acid Form: Base Form: Methy Violet: 0.0 - 1.6: 0.8: Yellow: Blue: Thymol Blue: 1.2 - 2.8: 1.6: Red: Yellow: Methyl Yellow: 29. Chloroform showed the highest affinity for bromothymol blue, an acid-base indicator ___ ( You be. Can be electrostatically immobilized on the surface on anion-exchanger resin like Amberlite IRA 401 and! Acid with pH 5 determination of the indicator were used diameter was used [. 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